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Subcutaneous and intramuscular in vivo oxygen sensing. Commercially available needle-type oxygen sensors <t>(PreSens)</t> were used to measure partial pressure of oxygen over time both (a) subcutaneously and (b) intramuscularly in 10- to 12-week-old nude mice. Sensors remained stationary and readings equilibrated after ~60 min to 7.4 and 3.0% in the subcutaneous and intramuscular regions, respectively. A temperature probe was used concomitantly to ensure the accuracy of the oxygen readings. The animal study was performed using a protocol (MO16A284) approved by The Johns Hopkins University Institutional Animal Care and Use Committee.
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Subcutaneous and intramuscular in vivo oxygen sensing. Commercially available needle-type oxygen sensors <t>(PreSens)</t> were used to measure partial pressure of oxygen over time both (a) subcutaneously and (b) intramuscularly in 10- to 12-week-old nude mice. Sensors remained stationary and readings equilibrated after ~60 min to 7.4 and 3.0% in the subcutaneous and intramuscular regions, respectively. A temperature probe was used concomitantly to ensure the accuracy of the oxygen readings. The animal study was performed using a protocol (MO16A284) approved by The Johns Hopkins University Institutional Animal Care and Use Committee.
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Subcutaneous and intramuscular in vivo oxygen sensing. Commercially available needle-type oxygen sensors <t>(PreSens)</t> were used to measure partial pressure of oxygen over time both (a) subcutaneously and (b) intramuscularly in 10- to 12-week-old nude mice. Sensors remained stationary and readings equilibrated after ~60 min to 7.4 and 3.0% in the subcutaneous and intramuscular regions, respectively. A temperature probe was used concomitantly to ensure the accuracy of the oxygen readings. The animal study was performed using a protocol (MO16A284) approved by The Johns Hopkins University Institutional Animal Care and Use Committee.
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World Precision Instruments needle type fiber optic oxygen microsensors
Subcutaneous and intramuscular in vivo oxygen sensing. Commercially available needle-type oxygen sensors <t>(PreSens)</t> were used to measure partial pressure of oxygen over time both (a) subcutaneously and (b) intramuscularly in 10- to 12-week-old nude mice. Sensors remained stationary and readings equilibrated after ~60 min to 7.4 and 3.0% in the subcutaneous and intramuscular regions, respectively. A temperature probe was used concomitantly to ensure the accuracy of the oxygen readings. The animal study was performed using a protocol (MO16A284) approved by The Johns Hopkins University Institutional Animal Care and Use Committee.
Needle Type Fiber Optic Oxygen Microsensors, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Subcutaneous and intramuscular in vivo oxygen sensing. Commercially available needle-type oxygen sensors <t>(PreSens)</t> were used to measure partial pressure of oxygen over time both (a) subcutaneously and (b) intramuscularly in 10- to 12-week-old nude mice. Sensors remained stationary and readings equilibrated after ~60 min to 7.4 and 3.0% in the subcutaneous and intramuscular regions, respectively. A temperature probe was used concomitantly to ensure the accuracy of the oxygen readings. The animal study was performed using a protocol (MO16A284) approved by The Johns Hopkins University Institutional Animal Care and Use Committee.
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Subcutaneous and intramuscular in vivo oxygen sensing. Commercially available needle-type oxygen sensors <t>(PreSens)</t> were used to measure partial pressure of oxygen over time both (a) subcutaneously and (b) intramuscularly in 10- to 12-week-old nude mice. Sensors remained stationary and readings equilibrated after ~60 min to 7.4 and 3.0% in the subcutaneous and intramuscular regions, respectively. A temperature probe was used concomitantly to ensure the accuracy of the oxygen readings. The animal study was performed using a protocol (MO16A284) approved by The Johns Hopkins University Institutional Animal Care and Use Committee.
Ph Microsensor Needle Type Fiber Optic Microsensor, supplied by PreSens Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Subcutaneous and intramuscular in vivo oxygen sensing. Commercially available needle-type oxygen sensors (PreSens) were used to measure partial pressure of oxygen over time both (a) subcutaneously and (b) intramuscularly in 10- to 12-week-old nude mice. Sensors remained stationary and readings equilibrated after ~60 min to 7.4 and 3.0% in the subcutaneous and intramuscular regions, respectively. A temperature probe was used concomitantly to ensure the accuracy of the oxygen readings. The animal study was performed using a protocol (MO16A284) approved by The Johns Hopkins University Institutional Animal Care and Use Committee.

Journal: Nature protocols

Article Title: O 2 -controllable hydrogels for studying cellular responses to hypoxic gradients in three dimensions in vitro and in vivo

doi: 10.1038/nprot.2017.059

Figure Lengend Snippet: Subcutaneous and intramuscular in vivo oxygen sensing. Commercially available needle-type oxygen sensors (PreSens) were used to measure partial pressure of oxygen over time both (a) subcutaneously and (b) intramuscularly in 10- to 12-week-old nude mice. Sensors remained stationary and readings equilibrated after ~60 min to 7.4 and 3.0% in the subcutaneous and intramuscular regions, respectively. A temperature probe was used concomitantly to ensure the accuracy of the oxygen readings. The animal study was performed using a protocol (MO16A284) approved by The Johns Hopkins University Institutional Animal Care and Use Committee.

Article Snippet: Microx TX3 microfiber-optic oxygen transmitter; temperature-compensated system (PreSens) Needle-type oxygen microsensors (PreSens) Manual micromanipulator (PreSens) 3D real-time imaging Confocal microscope (e.g., Carl Zeiss, model no. LSM 780) Cell tracking analysis Imaris (Bitplane, http://www.bitplane.com/download ) MATLAB (MathWorks, https://www.mathworks.com/products/matlab.html ) Prism (GraphPad, https://www.graphpad.com/scientific-software/prism/ ) Protease activity POLARstar OPTIMA plate reader (BMG Labtech) or similar fluorescent plate reader for wavelength measurements of fluorescent signals Cell proliferation assay UV/visible spectrometer (SpectraMax; Molecular Devices) RNA extraction NanoDrop spectrophotometer (Thermo Fisher Scientific, model no. ND-1000) Blood vessel perfusion Confocal microscope (Carl Zeiss, model no. LSM 780 Meta) General laboratory equipment Cell culture incubator Sterile 96-well plates for 3D cell culture Hemocytometer or cell counter Positive-displacement pipette and sterile capillary piston tips Water bath Heat block Sterile 1.5-, 2-, 15-, and 50-ml conical (micro)centrifuge tubes Glass beaker, 50 ml Spatula Forceps REAGENT SETUP !

Techniques: In Vivo